Cisplatin adducts
WebA) The cisplatin activation process occurs by exchange of one or two of its chlorides for water molecules (monoaquated and diaquated, respectively). B) Cisplatin can form covalent bonds with... WebCisplatin adducts are Guanine-Guanine (GG) DNA intrastrand cross links caused by the chemical compound Cisplatin. Cisplatin is used as a chemotherapy agent that contains …
Cisplatin adducts
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WebOct 23, 2003 · Cisplatin is a neutral inorganic, square planar complex that reacts with DNA to induce its characteristic biological effects, which culminate in either repair of the DNA damage and cell survival... WebCisplatin (NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP) is an inorganic platinum complex, which is able to inhibit DNA synthesis by …
WebMay 8, 2014 · The masses of the platinum adducts following digestion and separation by HPLC were measured by mass spectrometry. Our results demonstrate that hydrated electrons induce damage to thymines as well as detachment of the cisplatin moiety from both guanines in the oligonucleotide. WebMar 29, 2006 · Here we describe a new method to establish and characterize monoclonal antibodies (Mab) for structurally defined DNA adducts. The two major reaction products of cisplatin, the guanine-guanine (Pt- [GG]) and adenine-guanine (Pt- [AG]) intrastrand crosslinks are recognized by Mab R-C18 and R-B3, respectively.
WebNov 7, 2016 · Cisplatin, one of the most widely used anticancer drugs, crosslinks DNA and ultimately induces cell death. However, the genomic pattern of cisplatin-DNA … WebThese covalent adducts are formed by linking the 3, 4 ( pyrone) or 4', 5’ ( furan) edge of psoralen to the 5, 6 double bond of thymine. Psoralens can form two types of monoadducts and one diadduct (an interstrand crosslink) with thymine. [10] These adducts result in local distortions to DNA at the site of intercalation.
WebTherapeutic efficacy of cisplatin-based treatment of late stage urothelial carcinoma (UC) is limited by chemoresistance. To elucidate underlying mechanisms and to develop new approaches for overcoming resistance, we generated long-term cisplatin treated (LTT) UC cell lines, characterised their cisplatin response, and determined the expression of …
WebSep 24, 2024 · Cisplatin exerts anticancer activity via multiple mechanisms. Its most acceptable mechanism involves the formation of DNA–platinum adducts by interacting with purine bases, activating several signal transduction pathways, and silencing or activating several genes which finally leads to apoptosis. great turtle kayak tours discount codeWebDespite intense research efforts, no approved therapy is yet available. Cisplatin is a chemotherapeutic drug but induces acute tissue injury. Oleuropein (OLE) is a major … florida board of nursing lpnWebCisplatin is mainly eliminated via urine. The highest concentrations can be found in the liver, prostate and kidneys. Side effects include severe nausea, vomiting, ototoxicity and nephrotoxicity, together with myelosuppression (bone marrow suppression). great turtle mackinac islandWebIn general, combination of cisplatin with an antimetabolite, taxane, or topoisomerase inhibitor, can result in a modulation of platinum pharmacology on the DNA, for example, enhanced retention of the platinum-DNA adducts. These interactions are mostly sequence and cell type dependent. florida board of nursing home administratorWebJul 1, 2008 · Although oxaliplatin and cisplatin form similar adducts on DNA, cisplatin is a poor substrate for OCT1 and OCT2 compared with oxaliplatin and is not active against colorectal cancer. These properties suggest that OCT-mediated uptake of oxaliplatin into cancer cells is a major determinant of its activity. florida board of nursing oriWebCisplatin is an effective, widely used drug for many cancers, which shows a high level and broad spectrum of antitumor activities. (1−6) It has been known that the binding between this drug molecule and the DNA molecule disables the physiologic activities of DNA and eventually induces cell apoptosis. great turtle kayak tours mackinac island miWebJul 1, 2001 · Cisplatin is a widely used chemotherapeutic agent. It reacts with nucleophilic bases in DNA and forms 1,2-d(ApG), 1,2-d(GpG) and 1,3-d(GpTpG) intrastrand … great turnout