Edu ribobio

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August undefined, 2024

WebAfter 2 h of incubation with 50 M 5-ethynyl-20-deoxyuridine (EdU; RiboBio, China), the CPM cells were ﬁxed and stained with a C10310 EdU Apollo In Vitro Imaging Kit (RiboBio, China). We used. Cells 2024, 8, 360 4 of 13 a Leica DMi8 ﬂuorescent microscope to capture the EdU-stained cells with three randomly selected WebMar 13, 2024 · To investigate the proliferation of the cells, 50 μM 5-ethynyl-20-deoxyuridine was first added into cells to incubate for 2 h after 36 h of transfection (EdU) (RiboBio, China), with fixing with 4% paraformaldehyde (PFA) for 30 min and neutralizing with 4% PFA with 2 mg/mL glycine; then, they were permeabilized with 0.5% Triton X-100.

CircPTPRA promotes the progression of pancreatic ductal …

WebDetails. RiboBio Co. is a leading oligonucleotide-based biotech enterprise in China with the world-class R&D team and the cutting-edge technologies. The company develops and … WebApr 5, 2024 · The EdU (RiboBio, Guangzhou, China) reagent was added to each well at a final concentration of 50 μM for 1.5–2 h. Then, the cells were fixed with 4% paraformaldehyde solution and 0.5% Triton X-100 and subsequently incubated with apollo staining solution for 30 min at 25 °C and protected from light. Nuclei were lightproof … tpl ttl ppl hhl什么意思

Loss of m6A demethylase ALKBH5 promotes post‐ischemic …

WebEdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the … WebThe Invitrogen Click-iT EdU cell proliferation assays combine EdU labeling with powerful click chemistry to provide a superior alternative to traditional BrdU staining methods for detecting and quantitating newly synthesized DNA. Fast —Detection in as little as 80 min, without an antibody. Mild conditions —No denaturation required. WebFeb 28, 2024 · The coverslips were incubated with a primary antibody against EdU (RiboBio, Guangzhou, China) at 4 ℃ overnight, followed by a secondary antibody against Apollo488 for 1 hour at room temperature. Images were captured under a fluorescence microscope. Cell cycle analysis. Cell cycle was analyzed by flow cytometry. tpl traduction

IJMS Free Full-Text CircPLXNA2 Affects the Proliferation and ...

A novel 3-acyl isoquinolin-1(2H)-one induces G2 phase …

WebAverage 86 stars, based on 1 article reviews. Price from $9.99 to $1999.99. cell light edu apollo567 in vitro imaging kit - by Bioz Stars , 2024-04. 86 / 100 stars. Buy from Supplier. cell light tm edu apollo567 in vitro imaging kit ( Ribobio co ) 86. Ribobio co cell light tm edu apollo567 in vitro imaging kit. WebInvitrogen Click-iT EdU细胞增殖分析试剂采用click 化学技术，可为新合成的DNA检测与定量提供比传统BrdU方法更优秀的替代物。当在DNA合成过程中掺入修饰的核苷EdU（5-乙炔基-2'-脱氧尿苷）时，可以通过快速的“click化学”反应检测到它，并且对细胞的破坏作用最小。 tpl tote bagWebThe Click-iT EdU Cell Proliferation Kit for Imaging provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside … thermoskanne mit becher 1 l

"WebRibobio co edu cell proliferation detection kit Edu Cell Proliferation Detection Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more " - Edu ribobio

Edu ribobio

WebApr 14, 2024 · Objective: The current molecular classification system for gastric cancer covers genomic, molecular, and morphological characteristics. Non-etheless, classification of gastric cancer based upon DNA damage repair is still lacking. Here, we defined DNA damage repair-based subtypes across gastric cancer and identified clinicopathological, … WebMar 10, 2010 · DOI: 10.1016/j.brainres.2009.12.092. Abstract. Recently, a novel method for detection of DNA synthesis has been developed based on the incorporation of 5-ethynyl …

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WebThe cell proliferation after Gαi2 knockdown or overexpression was evaluated by Cell-Light™ EdU Apollo®488 In Vitro Imaging Kit (RiboBio) according to the manufacturer's instructions. Briefly, cells were labelled with 5-ethynyl-2′-deoxyuridine (Edu, RiboBio) at a final concentration of 50 μM for 2 h. WebRibobio co edu assay steps Edu Assay Steps, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

WebMay 1, 2024 · The Cell counting kit-8 (CCK-8) (Biotechwel, #WH1199) and EdU (RiboBio, #C10310-1) assays were performed according to the manufacturers' instructions. Briefly, after transfected with ALKBH5 siRNA or infected with the ALKBH5 adenovirus, CMECs were reseeded into a 96-well plate (5000 cells/ well), followed by the normoxic or hypoxic … WebEdU广泛应用于细胞增殖、分化、生长与发育、DNA损伤修复、病毒复制等方面的研究，尤其适合siRNA、miRNA、小分子化合物及药物的细胞增殖筛选实验。锐博生物具有EdU …

WebMay 12, 2024 · After treating with compound 4f for 48 h, cells were treated with 50 μM EDU (Ribobio, China), which was obtained through adding 1μL EDU primary liquid into 1 ml DMEM medium with 10% FBS and 1% penicillin-streptomycin according to manufacturer’s instruc-tions, for 2 h, and then fixed with 4% paraformaldehyde for 30 min at room … WebPhone Number 400-686-0075. RiboBio Co. is a leading oligonucleotide-based biotech enterprise in China with the world-class R&D team and the cutting-edge technologies. The company develops and supplies innovative DNA/RNA, RNAi and miRNA products, as well as provides diverse oligonucleotide related R&D services. RiboBio established a …

WebEdu Imaging Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol …

WebMar 10, 2010 · Recently, a novel method for detection of DNA synthesis has been developed based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, into cellular DNA and the subsequent reaction of EdU with a fluorescent azide in a copper-catalyzed [3+2] cycloaddition ("Click" reaction). … thermoskanne mit becherWebEdU细胞增殖检测. EU RNA转录活性检测. MTT细胞活性检测. 一步法TUNEL细胞凋亡检测试剂盒. 染色试剂. 一步法TUNEL细胞凋亡检测. Caspase-3/7细胞凋亡快速检测试剂盒 . 细胞器分离与分析. 外泌体分离与纯化 . 细胞转染. Small RNA转染. 转染指示剂 thermoskanne materialWebChongqing Medical University, Chongqing, 400016 China. Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400174 China. ... A biotin-labeled circPTPRA probe was designed and synthesized by RiboBio Co., Ltd. The RNA pull-down assay was performed according to the instruction of the Pierce™ … thermoskanne miluWebEdU (5-ethynyl-2’-deoxyuridine), a thymidine analog that is an alternative to BrdU, is also used in DNA proliferation assays that are simpler and faster than the BrdU assay. NB: EdU is also available as free molecule as ab146186 (EdU). In EdU staining, EdU is incorporated into newly synthesized DNA by cells within a sample. thermoskanne mit fotoWeb电话咨询: 4006860075 QQ 咨询: 4006860075 投诉电话: 020-32210536-643 投诉邮箱：[email protected] 4 / 6 以下是使用EdU/EU 标记后，选择本制品进行的染色实验方法。 Apollo 染色 3.1 加入100 μL的1X Apollo®染色反应液（表1），避光、室温孵育30 分钟后，弃染色反应液； thermoskanne mit becher testWebFigure Legend Snippet: A , proliferation assay via Cell-Light™ EdU DNA Cell Proliferation Kit showing decreased proliferation rate of MDA-MB-435 and SK-MES-1 cells treated with let-7b compared to the cells treated with random let-7b or control. The blue-stained cells were stained by Hoechst, and the red were EdU add-in cells. EdU-positive cells were … tpl ttl pplWeb广州市锐博生物科技有限公司（Guangzhou RiboBio Co., Ltd.）成立与2004年7月，由来自麻省大学医学院的张必良教授创办，位于风景美丽、环境优雅的广州科学城，国际企业孵化器内，是一家以siRNA化学合成、siRNA药物开发和技术支持服务为核心的生物高新技术企业。 tpl trust for public land