Flag-tag mouse monoclonal antibody

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August undefined, 2024

WebKey features and details PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt, Sandwich ELISA Reacts with: Species independent Conjugation: PE. Ex: 488nm, Em: 575nm Isotype: IgG1 Get better batch-to-batch reproducibility with a recombinant antibody WebThe FLAG™ Tag (peptide sequence DYKDDDK) is a short, ... The membrane was probed with DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 488 (Cat. No. MA1-142-A488) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging …

DYKDDDDK Tag Antibody (Binds to same epitope as …

WebHA-tag Rabbit Monoclonal Antibody staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. ... HA Tag Mouse Monoclonal Antibody. AG8050. 50μl. Flag Tag Rabbit … WebThe MA1-142 antibody (clone L5) has been successfully used as a detection antibody in a direct ELISA with FLAG-tagged protein-coated plates. Similar Epitope as the FLAG tag … lithographed toy fire trucks yonezaway

DYKDDDDK Tag Monoclonal Antibody (FG4R) - Thermo …

WebTHE™ DYKDDDDK Tag Antibody, mAb, Mouse THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins. Product Details Citations Documents Overview Applications Properties Examples Background For laboratory research use only. WebThe antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG ® antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. Species … WebDYKDDDDK Tag Monoclonal Antibody ... -Mouse Monoclonal Antibody Acquista DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin, Invitrogen™ su. Italiano English; Change Country. Ricevi tutte le ultime informazioni Centro di Assistenza e Supporto › Italiano English; Change Country; 0. No offer available; Struttura chimica ... ims property

3xFLAG® System Expression Vectors for Ultra-Sensitive …

A high-affinity monoclonal antibody against the FLAG tag …

WebPolyclonal Antibody for studying Flag-Tag. Cited in 708 publications. Validated for WB, IP, F. Available in 2 sizes. ... (G3A1) mAb IgG1 Isotype Control #5415 for mouse monoclonal IgG1 primary antibodies, Mouse … WebAntibodies that detect DYKDDDDK Tag can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Flow Cytometry. Our DYKDDDDK Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Rat, Goat and … ims propshaftsWebDetection was performed with ANTI-FLAG® M2 monoclonal primary antibody, anti-mouse-HRP secondary antibody, and ECL™ chemiluminescent substrate. Figure 3. Comparative Sensitivity of 3xFLAG®. Each protein fusion tag was cloned separately on the C terminal of GST. The purified proteins were diluted from 1 ug to 0.01 ng and analyzed. ims progressive outdoors

"WebJan 21, 2024 · For the primary antibody reaction, DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (1:1000, 14793; Cell Signal Technology, Beverly, MA, USA) and anti-C23 (NCL) mouse monoclonal (1:500, sc-55486; Santa Cruz Biotechnology, Dallas, TX, USA) were used in the antibody solution, and the mixture was allowed to react with the … " - Flag-tag mouse monoclonal antibody

Flag-tag mouse monoclonal antibody

A high-affinity monoclonal antibody against the FLAG tag

WebSpecificity: Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins. Immunogen: A synthesized peptide. Western blot analysis of Flag-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with Flag-tagged protein lysate. β-Actin Rabbit Polyclonal Antibody WebMonoclonal ANTI-FLAG ® M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells. …

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WebFlag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the … WebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived …

WebMouse monoclonal [F-tag-01] antibody to DDDDK Tag. Applications: ICC, WB: Reactivity: Species independent: Conjugate: Unconjugated: ... Anti-FLAG Tag Antibody [FG4R] … WebFLAG Tag Rabbit Polyclonal Antibody: 100μl: AF5051: Flag Tag Mouse Monoclonal Antibody: 50μl: AF519: Flag抗体(小鼠单抗) >40次: AF0033: Myc Tag Mouse Monoclonal Antibody: 100μl: AF5054: Myc Tag Mouse Monoclonal Antibody: 50μl: AF6513: c-Myc Rabbit Polyclonal Antibody: 50μl: AM926:

WebKey features and details. PerCP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt. Reacts with: Species independent. Conjugation: PerCP. Ex: 482nm, Em: 675nm. Isotype: IgG1. WebFlag Tag Monoclonal Antibody Datasheet Product Details Citations Customer Reviews and Q&A Product Details Citations Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth SC Wu,Nature communications,2024

WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence.

WebMonoclonal ANTI-FLAG®M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.colicells. Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. ims project trackerWebConfocal image showing positive staining for FLAG® on HEK-293T cells transfected with DDDDK-tagged PFKFB3 expression vector. Mouse … ims property groupWebProduct Specific Information. This antibody detects Flag tag from various samples. similar epitope as the FLAG tag from Sigma. MA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications. The MA1 … The membrane was probed with a DyLight 800-conjugated FLAG Epitope Tag … Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1 … lithographer famousWebJun 15, 2012 · The FLAG sequence (DYKDDDDK) is an artificial sequence widely used to detect, quantify, and purify proteins expressed as FLAG-fusion proteins. Several highly specific monoclonal antibodies for FLAG are commercially available; however, they are not always sensitive enough to detect proteins expressed … ims property services ims property solutionsWebHost Species Mouse; Clonality Monoclonal; Purification Affinity purification using immunogen. Applications WB,IF IP; Species Reactivity Hu Ms Rt; Specificity The Flag … ims promotional productsWebDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) DDDDK tag Monoclonal Antibody for ELISA. Host / Isotype. Mouse / IgG2b. Reactivity. human, mouse, rat . ... Monoclonal ANTI-FLAG? M2 … lithographer computer