Hct116 p53+/+ cells
WebJan 5, 2024 · The p53-null HCT116 p53 (-/-) cells were transduced HCT116 stable expressing p53 R273H and p53 R248W cell lines and were generated by transduction … WebAug 8, 2014 · What is the p53 deletion in HCT116 p53-/- cells? In our current project we are using the HCT116 p53-/- cells originally created by homologous recombination by Bunz …
Hct116 p53+/+ cells
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WebJan 17, 2006 · An increase in Ser15P-detected activity shows that P53 was stabilized in cell lines with WT p53: HCT116, LS180, SW48, and NCI-747. There was either no change in … WebHCT116 cells with p53 wild-type and p53 null-type characteristics and HT29, DLD-1 cells were seeded in 96-well plates (1 × 10 4 cells/well) and ES were treated with each concentration for 24 h. Following treatment, MTT (2 mg/mL) was added to each well to have a final concentration of 0.5 mg/mL, and then, dimethyl sulfoxide (DMSO) was treated ...
HCT116 cells have a mutation in codon 13 of the KRAS proto-oncogene, and are suitable transfection targets for gene therapy research. The cells have an epithelial morphology and can metastasize in xenograft models. When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in … See more HCT116 is a human colon cancer cell line used in therapeutic research and drug screenings. See more HCT116 cells are used in a variety of biomedical studies involving colon cancer proliferation and corresponding inhibitors. The cell line has been used in tumorigenicity studies, along with other research that has shown that Cyclin D1 holds large … See more • Cellosaurus entry for HCT116 See more WebApr 7, 2024 · F Knockdown of DDX21 attenuates HCT116 cell ... Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function. Nat Cell Biol. 2024 ...
WebJul 3, 2013 · HCT116 p53 +/+, HCT116 p53 –/– cells, obtained from P. Hainaut (Lyon, France) were ascertained at thawing based on their differential expression of p53 and p21. HCT116 cells being highly unstable, only early-passage isolates (maximum of five) were used, and all key functional data were confirmed with another cell line (LS513).
WebFurthermore, downregulation of JMJD2D inhibited cell proliferation in wild-type and even more so in p53(-/-) HCT116 colon cancer cells, suggesting that JMJD2D is a pro …
WebJul 31, 2024 · C HCT116 p53 WT and p53 KO cells were transfected as in (A) and cell cycle analyses were performed 72 h later. Bar chart shows mean values ± SD of three biological replicates ( n = 3). once ready-to-showWebHNK affects stem cell marker expression. (A) HCT116 cells were grown in spheroid growth media in low adherent plates and treated with 25 µM HNK. After 5 days, the colonospheres were photographed and counted. ... Lysates from HNK treated cells show an increase in p53 phosphorylation and PUMA expression. (C) Cytoplasmic extracts from HNK treated ... once publishedWebSep 9, 2024 · Cells (HCT116 WT, HCT116 p53 –/–, MCF7, and U2OS) were cultured at 37°C with 5% CO 2 in DMEM high glucose (Hyclone) supplemented with 5% fetal bovine serum (FBS, Hyclone) and penicillin–streptomycin. All cells used were obtained from ATCC, which performs short-tandem repeat profiling to confirm the identity of their cell lines. oncept tokyoWebApr 13, 2024 · Cells treated with mannose/5-FU showed a significant cell accumulation in the sub-G 1 region in a time-dependent manner in HCT116 and HCT116 p53 −/− but not … once pythonWebJul 20, 2024 · Isogenic human colorectal cancer cell lines HCT116 (p53 +/+ and p53-/-) were irradiated with high-LET carbon ions. Cell survival was determined by the standard … once receivingWebJul 22, 2024 · Furthermore, STAT3 phosphorylation and c-Myc expression were diminished in HCT116 (p53 +/+) but not in HCT116 (p53 −/−) or CaCO 2 cells after flavopereirine treatment, indicating P53 is upstream of JAKs-STATs-c-Myc signaling. P53 upregulation by the flavopereirine-DNA complex might be largely responsible for the suppression of CRC … once put itWebJun 10, 2016 · Three biological replicates were used for p value, p*<0.01. (H,I) NGFR mRNA expression is inhibited by p53 knockdown. HCT116 p53+/+ (H) and H460 (I) cells were transfected with p53 or control siRNA for 72 hr, and Doxorubicin or 5-Fluorouracil was supplemented 15 hr before the cells were harvested for q-PCR (Mean ± SEM, n = 3). … oncept phoenix